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KMID : 0351619640050010049
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Kyungpook Medical Journal
1964 Volume.5 No. 1 p.49 ~ p.66
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Studies on Antigenicities of Protein Fraction(CPF) Extrscted from Adult Worms of Clonorchis sinensis
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Choi Dong-Wik
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Abstract
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A protein fraction of the adult Clonorchis sinensis (CPF) was extrated, and its antigenicities were studied by means of intradermal and precipitation reaction on the rabbits and human subjects. Rabbits used in this study were divided into three groups; the first group was experimentally infected with metacercaria of Clonorchis sinensis (infected rabbit), the second group sensitized with CPF (sensitized rabbit), the third group served as control (non-infected rabbit). The humen subjects vas also divided into groups; the first the patient of clonorchiasis, the second the control.
Simultaneously, a protein fraction was also extracted from the adult worms of Paragonimus westermani (PPF) and its antigenicity was studied on the human patients of paragonimiasis and controls. Furthermore, the cross reaction of antigenicity between these two different Elul; infestation was studied. The results obtained were as follows;
1) In the infected rabbits, the wheal of the intradermal reaction reached the maximum size in 50 minutes and erythema in EO minutes after the intracutaneus injection of the CPF antigen.
2) In the sensitized rabbit group, wheal and erythema of the intradermal reaction reached maximal size in 60 minutes.
3) In the control group, only wheal was produced following antigen injection, which appeared in 10 minutes and sustained also for 10 minutes, then disappeared in 70 minutes.
4) The intradermal reaction, in the infected rabbits, turned positive on the thirtieth day of experimental infection, reaching maximum size of wheal (57mm.) in 30 days and maintained average diameter of 5 to 6mm. thereafter. Theprecipitation began to show positive (1:1000 to 1 :2CJ0) on the fourtieth day of experimental infection, teaching peak level (1:4000) in 100 days, and maintained titer at 1:2000 antigen dilution thereafter. The two reactions were parallel in reactivity from the 85 to 1COth day after injection.
5) The sensitized rabbits began to show positive precipitation rcactien on the fifth day of sensitization, reaching maximum (1:16000 to 1 : 32JC0) in 10 to 20 days, then it decreased rapidly initially and slowly later. The intradermal reaction turned positive on the fifth day: the reactivity increasing while the precipitation decreasing between the 25th and 40th day, and former reaction reached the peak on the 85th to 130th day.
6) In the patients of the clonorchiasis, the i~ositivity of intradermal reaction was more mar-led than that of precipitation, and no correlation was found between these two reactions.
7) The results of the both reactions by PPF antigen in paragonimiasis was resamble as above 6), but generally, all positivities was higher than above 6).
8) In the results of precipitation and intrader-rr~.al reaction which were reciprocally tested with serum of clonorchiasis patient by PPF antigen arrd with serum of paragonimiasis patient by C]?F antigen, there were many cross reactions and nospecificity.
9) In human subjects, there was no doss eor-rel ation between the intradermal and precipitation reactions, although intradermal reactions were mostly positive in the case of positive precipitations.
10) When bout the CPF and PPF antigen were given intracutaneously cn the same human patient of clonorchiasis or paragonimiasis, the formerantigen produced the larger wheal ir. clonorchiasis, while the latter produced also the lar;;cr wheal in paragonimiasis as expected.
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